cells culture human primary osteoblasts hob  (Cell Applications Inc)

Journal: Journal of biomedical materials research. Part A

Article Title: In-Vitro and In-Vivo Biocompatibility Assessment of Free Radical Scavenging Nanocomposite Scaffolds for Bone Tissue Regeneration

doi: 10.1002/jbm.a.36816

Figure Lengend Snippet: Representative scanning electron micrographs of cell attachment to PT, PTC10, and PTC20 scaffolds after 7 days of culture. (a-c) Scaffolds with no cells were used as a reference for no attachment. Attachment of (d-f) human osteoblast cells to the surface of nanocomposites highlighted by arrowheads.

Article Snippet: Osteoblast Cell Culture and Biocompatibility Human Osteoblast Cell Culture Primary human osteoblast cells (HObs) purchased from Cell Applications Inc. (San Diego, CA) were used to further assess the cell interactions between osteoblast cells and nanocomposite scaffolds.

Techniques: Cell Attachment Assay

Journal: Journal of biomedical materials research. Part A

Article Title: In-Vitro and In-Vivo Biocompatibility Assessment of Free Radical Scavenging Nanocomposite Scaffolds for Bone Tissue Regeneration

doi: 10.1002/jbm.a.36816

Figure Lengend Snippet: In-vitro biocompatibility of human osteoblasts on nanocomposite scaffolds. (a) Viability of HOb cells seeded onto scaffolds (n=6). (b) Quantification of DNA to determine cell proliferation and growth on scaffolds over 35 days. (c) Quantification of ARS stain to measure mineralization by HObs over 0, 14, 28, and 35 days. Averages were reported as mean ± standard deviation, statistically significant change is denoted by the * symbol.

Article Snippet: Osteoblast Cell Culture and Biocompatibility Human Osteoblast Cell Culture Primary human osteoblast cells (HObs) purchased from Cell Applications Inc. (San Diego, CA) were used to further assess the cell interactions between osteoblast cells and nanocomposite scaffolds.

Techniques: In Vitro, Staining, Standard Deviation

Journal: International Journal of Nanomedicine

Article Title: Atomic layer deposition of nano-TiO₂ thin films with enhanced biocompatibility and antimicrobial activity for orthopedic implants

doi: 10.2147/ijn.s148065

Figure Lengend Snippet: Figure 10 Osteoblast proliferation on Ti and Ti-TiO2 (160°C) samples. Data represent mean ± SD. N=3. *p,0.05 compared with Ti at the same time period, **p,0.05 compared with the same sample (Day 1). Abbreviations: Ti, titanium; TiO2, titanium dioxide.

Article Snippet: Casein protein has been shown to be a key protein in TSB medium (17 g casein/L); thus, to quantify casein protein adsorption, we incubated samples with the prepared 1.7% by weight casein solution (17 g casein/L H 2 O, Sigma-Aldrich), and then the same procedures, as already described, to determine total protein adsorption were used. cell assays cell culture Human osteoblasts (PromoCell, C-12720) and human dermal fibroblasts (Lonza, CC-2509) at population numbers less than 10 were used for all cell experiments.

Techniques: Titanium Dioxide

Journal: International Journal of Nanomedicine

Article Title: Atomic layer deposition of nano-TiO₂ thin films with enhanced biocompatibility and antimicrobial activity for orthopedic implants

doi: 10.2147/ijn.s148065

Figure Lengend Snippet: Figure 9 Osteoblast adhesion on Ti samples with different TiO2 coatings after 4 hours of culture. Data represent mean ± SD, N=3. *p,0.05 compared with Ti control (labeled as Ti). Abbreviations: Ti, titanium; TiO2, titanium dioxide.

Article Snippet: Casein protein has been shown to be a key protein in TSB medium (17 g casein/L); thus, to quantify casein protein adsorption, we incubated samples with the prepared 1.7% by weight casein solution (17 g casein/L H 2 O, Sigma-Aldrich), and then the same procedures, as already described, to determine total protein adsorption were used. cell assays cell culture Human osteoblasts (PromoCell, C-12720) and human dermal fibroblasts (Lonza, CC-2509) at population numbers less than 10 were used for all cell experiments.

Techniques: Control, Labeling, Titanium Dioxide

Journal: International Journal of Nanomedicine

Article Title: Atomic layer deposition of nano-TiO₂ thin films with enhanced biocompatibility and antimicrobial activity for orthopedic implants

doi: 10.2147/ijn.s148065

Figure Lengend Snippet: Figure 12 (A) Osteoblast and (B) fibroblast cell density (after 4 hours of culture) was directly proportional to the surface energy on Ti controls and Ti-TiO2 samples. Error bars represent SD. Abbreviations: Ti, titanium; TiO2, titanium dioxide.

Article Snippet: Casein protein has been shown to be a key protein in TSB medium (17 g casein/L); thus, to quantify casein protein adsorption, we incubated samples with the prepared 1.7% by weight casein solution (17 g casein/L H 2 O, Sigma-Aldrich), and then the same procedures, as already described, to determine total protein adsorption were used. cell assays cell culture Human osteoblasts (PromoCell, C-12720) and human dermal fibroblasts (Lonza, CC-2509) at population numbers less than 10 were used for all cell experiments.

Techniques: Titanium Dioxide